Considerations To Know About cytometers
Considerations To Know About cytometers
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Facts-enriched characterization of mobile populations. Morphological info from images can include towards the richness of flow cytometry knowledge. By way of example, the determine exhibits an in any other case regular apoptosis assay working with Annexin V and PI, including cell imaging to characterize cells in Every populace to expose morphologically distinctive characteristics. These insights could not happen to be received from multiplex staining by itself.
As samples are obtained around the Attune CytPix Flow Cytometer, the higher-speed brightfield digicam captures and suppliers photos of detected activities at a amount around 6,000 photographs for every 2nd, depending on the flow level and graphic measurement. For larger adaptability, Attune Cytometric Software permits you to modify the impression capture frequency as needed and select a specific gate for graphic capture, if essential.
(B) RAW 264.7 macrophages are often annotated inaccurately with regard to particle count due to their complexity and noncircular borders. The volume of singlets accessible for Evaluation raises when the user trains and employs a far more exact design.
Discover new insights into your experimental facts by visualizing populations applying put together fluorescence and morphology facts. Our computer software interface wizards allow downsampling, concatenation (merged data files) and dimensionality applications which might be constructed into your software package for an intuitive, streamlined information Assessment encounter.
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Here, a skilled user has gated singlets confidently. Just after assessing the manual singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate is made up of more than four% aggregates.
Pair your Attune flow cytometer with the autosampler for greater workflow performance, and add robotic integration for optimum throughput.
Consistent graphic excellent even at large throughput Acoustic concentrating plus a superior-speed camera Merge to graphic these T cells continually at very low or superior flow prices. Quickly change target and camera options to meet experimental specifications.
Early signs of this contamination had originally been dismissed as particles. Backgating to discover new populations. Backgating imaged cells about the Attune CytPix Flow Cytometer also enables you to use morphological functions to find out fascinating subpopulations that would not be obvious from flow cytometry facts by itself.
Possibly most significantly, these situations comprise cells of Plainly diverse phenotypes which could lead to incorrect conclusions about double beneficial functions (especially in rare populations).
AI-pushed automated impression Examination Using the Attune Cytometric Computer software translates party characteristics into distinct morphology parameters that may be coupled with conventional fluorescence and scatter parameters to expose really reproducible inhabitants alterations and exclusive subsets.
We Beforehand demonstrated the power of imaging CAR-T/Ramos mobile interactions. Enable’s take a look at just the inhabitants of best interest, the double positive occasions, To find out more. We could now use extended morphological parameters (here, circularity and skewness of intensity) to additional take a check here look at the attributes of such populations and refine gating on these events, rising facts robustness.
Leverage the power of Superior engineering and innovation to accelerate your discoveries. Have confidence in within the Attune Flow Cytometer spouse and children to provide the dependability, pace, and precision that established your operate apart.
No wash/no lyse leukocyte analysis with imaging. Cells had been obtained from 24-hour-outdated blood by dilution in 1 mM EDTA ( Expert UX/UI enables consumers to prepare AI products for expanded purposes. (A) The “Practice Product” aspect gives annotation equipment and serious-time design teaching precision.
At the guts of each and every Attune Flow Cytometer lies our innovative acoustic focusing know-how, which utilizes sound waves to meticulously align cells in the flow cell. This modern strategy surpasses traditional hydrodynamic procedures, resulting in quicker processing speeds, Fantastic data high quality, and elevated sample throughput.
The Attune Xenith cytometer is a significant-parameter spectral analyzer, furnishing both equally spectral unmixing and conventional flow abilities for in depth and functional mobile Examination.
Agilent Worth Assure assures you at the least a decade of instrument use within the day you purchase. Usually, We're going to credit history you with the residual value of the process toward an upgraded design.
What appeared to be a homogeneous population is definitely much more diverse—an Perception that ought to be viewed as when interpreting results.